Spectroscopic Characterisation of an Aconitase (AcnA) of Escherichia coli
A spectroscopic study of an aconitase, AcnA, from Escherichia coli is presented. The amino acid sequence of AcnA has 53% identity with mammalian cytosolic aconitase (c-aconitase) which is the...
View ArticleThe Periplasmic Nitrate Reductase of Thiosphaera pantotropha
The aerobically-active periplasmic respiratory nitrate reductase (Nap) from the bacterium Thiosphaera pantotropha is a heterodimer of NapA (90kDa), which binds molybdopterin guanine dinucleotide (MGD)...
View ArticleReconstitution of the [4Fe-4S] Cluster in FNR and Demonstration of the...
The FNR protein of Escherichia coli is a redox-responsive transcription regulator that activates and represses a family of genes required for anaerobic and aerobic metabolism. Reconstitution of...
View ArticleStructural Investigation of the Molybdenum Site of the Periplasmic Nitrate...
The molybdenum centre of the periplasmic respiratory nitrate reductase from the denitrifying bacterium Thiosphaera pantotropha has been probed using molybdenum K-edge X-ray absorption spectroscopy....
View ArticleDimethylsulfoxide Reductase: Insight into the Mechanisms of the Catalytic...
Dimethysulfoxide (DMSO) reductase from the photosynthetic bacteria Rhodobacter sp. is a molybdenum containing enzyme belonging to the structurally and catalytically diverse family of non-nitrogenase...
View ArticleMultiple States of the Molybdenum Centre of Dimethylsulphoxide Reductase from...
The dimethylsulphoxide reductase of Rhodobacter capsulatus contains a pterin molybdenum cofactor molecule as its only prosthetic group. Kinetic studies were consistent with re-oxidation of the enzyme...
View ArticleMo(V) Electron Paramagnetic Resonance Signals from the Periplasmic Nitrate...
A Mo(V) electron paramagnetic resonance (EPR) study of the periplasmic respiratory nitrate reductase of the denitrifying bacterium Thiosphaera pantotropha has revealed that the molybdenum centre of...
View ArticleHomoleptic Nickel(II) Complexes of Redox-Tunable Pincer-type Ligands
Different synthetic methods have been developed to prepare eight new redox-active pincer-type ligands, H(X,Y), that have pyrazol-1-yl flanking donors attached to an ortho-position of each ring of a...
View ArticleIdentification of Periplasmic Nitrate Reductase Mo(V) EPR Signals in Intact...
EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the...
View ArticleChemical Switching Behaviour of Tricarbonylrhenium(I) Complexes of a New...
The structures and optoelectronic properties of tricarbonylrhenium(I) complexes of di(2-pyrazolyl-p-tolyl)amine in its neutral and deprotonated (uninegative amido) form were investigated. Reactions of...
View ArticleEPR of Cobalt-Substituted Zinc Enzymes
Co(II) is sometimes utilized as a spectroscopically active substitute for Zn(II) in enzymes. Metal binding sites in enzymes that contain catalytically active Zn(II) generally yield high-spin S = 3/2...
View ArticleA Deep Chandra ACIS Survey of M83
We have obtained a series of deep X-ray images of the nearby galaxy M83 using Chandra, with a total exposure of 729 ks. Combining the new data with earlier archival observations totaling 61 ks, we...
View ArticleConformational Dynamics of metallo-β-lactamase CcrA during Catalysis...
Previous crystallographic and mutagenesis studies have implicated the role of a position-conserved hairpin loop in the metallo-β-lactamases in substrate binding and catalysis. In an effort to probe...
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